Journal of Agriculture and Ecology Research International <p style="text-align: justify;"><strong>Journal of Agriculture and Ecology Research International (2394-1073)</strong> aims to publish high quality papers (<a href="/index.php/JAERI/general-guideline-for-authors">Click here for Types of paper</a>) in all areas of Agriculture and Ecology. The journal also encourages the submission of useful reports of negative results. This is a quality controlled, OPEN peer reviewed, open access INTERNATIONAL journal.</p> SCIENCEDOMAIN international en-US Journal of Agriculture and Ecology Research International 2394-1073 Incidence and Distribution of Rice Yellow Mottle Virus Disease in Kebbi State, Nigeria <p>The study was conducted to assess the incidences of Rice Yellow Mottle Virus disease (RYMVD) in Kebbi State Nigeria, a field survey was conducted in four rice-growing areas of the State. Rice fields were selected randomly at 2 km interval, severity of the disease was assess using arbitrary five-point scale and disease incidence was assessed according to the proportion of the plants showing symptoms. Thirty plants were assessed in each field visited. Symptoms occurred in varying levels of incidence. The presence of RYMV in the collected samples was confirmed using Reverse Transcription Polymerase Chain Reaction (RT-PCR). Mottle/yellowing symptom was found more on the plants assessed (46%) followed by leaf curling (21%), leaf necrosis (09%), leaf deformation (11%) and irregular patches (13%). RYMVD was found highly distributed in the State with average incidence of 54.38%. The highest incidence was recorded in in Yauri (67.50%) followed by Argungu (55.00%), Bagudo (52.50%) and the lowest was recorded in Suru (42.50%). The average symptom severity across all the four Local Governments visited was 2.8, the highest was recorded in Yauri (3.2), followed by Argungu (2.9), Bagudo (2.7) and Suru 2.3. The information obtained in this study would assist rice breeding programs to develop durable RYMV rice resistant cultivars and guide in the identification of RYMVD hot spot locations for seed multiplication trials in Kebbi State.</p> I. U. Mohammed Y. A. Busari A. Muhammad R. Idris M. Adamu A. A. Ajala M. A. Yakub A. S. Muhammad ##submission.copyrightStatement## 2019-10-25 2019-10-25 1 7 10.9734/jaeri/2019/v20i1-230094 Evaluation of Four Species of Wild Yams, as Potential Natural Reservoirs of Potyviruses Infecting Yams Cultivated in Togo <p>Yams cultivation in Togo is hampered by diseases caused by Potyviruses, mainly <em>Yam mosaic virus</em> (YMV) and <em>Yam mild mosaic virus</em> (YMMV). To understand the Potyviruses dissemination mechanism and to develop an efficient control method, the present study aims to establish the role of wild yams species as potential natural reservoirs of these pathogens. As such, Potyvirus susceptibility assessment was performed on four wild yams, <em>D. dumetorum</em>, <em>D. bulbifera</em>, <em>D. togoensis </em>and, <em>D. smilacifolia</em>, which grow spontaneously in yam fields in Togo. For this, phytosanitary surveys were carried out on yam fields and forests near yam plots, in July 2018 at the long rainy season, covering 27 localities in Maritime, Central and Plateaux regions of Togo, during which wild yam leaves were sampled for viruses identification. The leaves samples were analyzed first by ACP-ELISA test to detect Potyviruses using universal anti-potyvirus monoclonal antibodies, and then by RT-PCR test to identify YMV and YMMV, using respectively pairs of primers YMV1&amp;YMV2 (196 pb) and YMV-CP-2F &amp; YMV-UTR-1R (249 pb). Then 140 seedlings obtained from seeds of the four wild yams, were inoculated with YMV isolate 20-601/06. ACP-ELISA test revealed that only the leaves samples of <em>D. dumetorum</em> and <em>D. togoensis</em>, collected in Plateaux region, were infected by Potyviruses, with respectively 24.24% and 6.25% of incidence rate. But these samples were positive for neither YMV nor YMMV at RT-PCR test. However, after the inoculations, respectively 20% of seedlings of <em>D. dumetorum</em>, 52.5%<em> of D. bulbifera</em>, 64%<em> of D. togoensis,</em> and 3.33%<em> of D. </em><em>smilacifolia</em>, were infected by YMV<em>. </em>This suggests a high potential of these yams, mostly<em> D. bulbifera</em> and <em>D. togoensis,</em> to become natural reservoirs for YMV, under high pressure of the viruses and their vectors. These wild yams control in and around yam fields can help limit Potyviruses infections.</p> Kwasi Dzola Ayisah Mawuli Kossivi Aziadekey Yawovi Mawuena Dieudonné Gumedzoe ##submission.copyrightStatement## 2019-11-16 2019-11-16 1 9 10.9734/jaeri/2019/v20i130095