Evaluation of Potential Accessions of African Rice (Oryza glaberrima Steud) with Essential Mineral Elements for Breeding Purposes
H. M. Amoatey
Nuclear Agriculture Centre, Biotechnology and Nuclear Agriculture Research Institute, Ghana Atomic Energy Commission, P.O.Box LG 80, Legon-Accra, Ghana and Department of Nuclear Agriculture and Radiation Processing, School of Nuclear and Allied Sciences, University of Ghana, P.O.Box AE 1, Atomic Energy, Kwabenya- Accra, Ghana
H. A. Doku *
Department of Biotechnology, Seed and Food Science Division / Rice, Council for Scientific and Industrial Research, Crops Research Institute, P.O.Box 3785, Fumesua-Kumasi, Ghana.
K. Nyalemegbe
Department of Agriculture Economics and Agribusiness, Evangelical Presbyterian University College, P.O.Box HP 678, Ho, Ghana.
D. Bansa
Nutrition Research Centre, Radiological and Medical Sciences Research Institute, Ghana Atomic Energy Commission, P.O.Box LG 80, Legon-Accra, Ghana.
*Author to whom correspondence should be addressed.
Abstract
Aim: The study was to identify superior accessions of African rice (Oryza glaberrima Steud.) for breeding to obtain nutrient-rich varieties to combat micro-nutrient malnutrition.
Place and Duration of Study: Field experimentation was carried out at Soil and Irrigation Research Institute, University of Ghana. Laboratory analyses were done at Ghana Research Reactor 1 (GHARR-1) and the Department of Chemistry and Environmental Research Centre, both of the National Nuclear Research Institute, Ghana Atomic Energy Commission. All the research activities were conducted between the period July, 2010 and June, 2011.
Methodology: Seventeen local accessions of African rice were evaluated for their reproductive and yield characteristics alongside NERICA 1, a high-yielding, protein-rich hybrid purposely bred for Africa. The dried seeds were analyzed for seven essential mineral elements: calcium, copper, iron, magnesium, manganese, potassium and zinc using atomic absorption spectrophotometry and neutron activation analysis.
Results: The study did not identify any accession with high or appreciable levels of both iron and zinc which are considered vital in alleviating micro-nutrient malnutrition. However, the accession N/4 showed significantly (P < 0.05) high zinc content (30.10 mg kg-1) while Awerema significantly (P < 0.05) recorded the highest amount of iron (386.6 mg kg-1).
Conclusion: The two accessions may therefore be utilized in a hybridization program for developing new lines of African rice rich in both iron and zinc.
Keywords: Atomic absorption spectrophotometry, biofortification, genetic diversity, micronutrient mal-nutrition, neutron activation analysis, Oryza glaberrima