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Aims: The aim of this study was to establish the effect of selected soil amendments on Ralstonia solanacearum isolates in greenhouse on selected solaneceous crops.
Study Design: The study was laid out as randomized complete block design (RCBD) in split pot arrangement for two seasons in the greenhouse.
Place and Duration of Study: The experiment was carried out in Kenyattta University situated in Kiambu County about 20 km from Nairobi city along Nairobi-Thika road between July, 2017- September, 2017 and between November, 2017- January, 2018.
Methodology: The three host crops of interest (potatoes, tomatoes and capsicum) were inoculated with prepared pure bacterial isolates; 18 (2T-Kiambu-Low Land), 71(2A-Nyeri-Low Land), 67 (2A-Nyeri-High Land), 83 (2T-Kirinyaga-Highland) and MX (18/71/67/83). Potatoes, tomatoes and capsicum were planted in pots each with a radius of 0.07 m (area 0.015 m2).The experiment had a total of 450 pots having a total area of 6.93 m2. The treatments were ChalimTM, Super-hydro-grow polymer + Metham sodium, Metham sodium, Metham sodium & Orange peel, Super-hydro-grow polymer, Brassica tissues, ChalimTM + Super-hydro-grow polymer, Brassica tissue + Orange peel, Metham sodium + Super-hydro-grow polymer and Control (no amendments).
Results: There were significant differences (P≤0.05) in the bacterial wilt incidences in selected solaneceous crops between control and all the soil amendments used in season 1 and 2. Brassica tissue + Super-hydro-grow polymer was superior in reducing bacterial wilt incidences in selected solaneceous crops in all the R. solanacearum isolates from Kenyan highlands and lowlands both in season 1 and 2.
Conclusion: Organic and inorganic soil amendments could serve as a viable control of bacterial wilt in solaneceous crops caused by R. solanacearum in the greenhouse.
Godfray HCJ, Pretty J, Thomas SM, Warham EJ, Beddington JR. Linking policy on climate and food. Science. 2011; 331:1013–1014. (DOI:10.1126/science.1202899)
Devendra KC, Aundy K, Sajad N. In-vitro Evaluation of Arabidopsis thaliana Ecotypes against Ralstonia solanacearum Race4, Int. J. Curr. Microbiol. App. Sci. 2017;6(5):575-579.
Guidot A, Coupat B, Fall S, Prior P and Bertolla F. Horizontal gene transfer between Ralstonia solanacearum strains detected by comparative genomic hybridization on microarrays. ISME J. 2009;3:549–5
Prior P, Ailloud F, Dalsing BL, Remenant B, Sanchez B, Allen C. Genomic and proteomic evidence supporting the division of the plant pathogen Ralstonia solanacearum into three species. BMC Genom. 2016;17(90).
Wei Y, Moreno CC, Gongora TJ, Wang K, Sang Y, Duran R, Macho AP. The Ralstonia solanacearum csp22 peptide, but not flagellin-derived peptides, is perceived by plants from the Solanaceae family. J Plant Biotech. 2018;1–14.
Sikirou R, Zocli B, Paret ML, Deberdt P, Coranson-Beaudu R, Huat J. First report of bacterial wilt of Gboma (Solanum macrocarpon) caused by Ralstonia solanacearum in Benin. Plant Dis. 2015; 11:1640–1640.
Singh D, Sinha S, Yadav DK, Chaudhary G. Detection of Ralstonia solanacearum from asymptomatic tomato plants, irrigation water, and soil through non-selective enrichment medium with hrp gene-based bio-PCR. Curr Microbiol. 2014;69:127- 134.
Jiang Y, Li B, Liu P, Liao F, Weng Q, Chen Q. First report of bacterial wilt caused by Ralstonia solanacearum on fig trees in China. For. Pathol. 2016;46:256–258. DOI:10.1111/efp.12267
Tim M, Prakash P, Carlos L. North Florida Research And Education Center (Nfrec), Quincy; Prakash Pradhanang Post-Doctoralassociate, Nfrec, Quincy, Fl 32351; Carlos A. Lopes, Researcher, Embrapa Hortalicas, Brasilia 70359-970, Brasil; 2008.
Joshi M, Srivastava R, Sharma AK and Prakash A. Screening of Resistant Varieties and Antagonistic Fusarium oxysporum for Biocontrol of Fusarium Wilt of Chilli. J Plant Pathol Microb. 2012; 3:134.
Muthoni J, Hussein S, Melis R. Management of bacterial wilt Ralstonia solanacearum Yabuuchi et al. of Potatoes: Opportunity for Host Resistance in Kenya Journal of Agricultural Science. 1995;4(9).
Meng F. The virulence factors of the bacterial wilt pathogen Ralstonia solanacearum. J Plant Pathol Microbiol. 2013;4(3).
Swanson JK, Yao J, Tans‐Kersten J, Allen C, Behavior of Ralstonia solanacearum race 3 biovar 2 during latent and active infection of geranium. Phytopathology. 2005;95:136–43.
Genin S, Boucher C. Ralstonia solanacearum: secrets of a major pathogen unveiled by analysis of its genome. Mol Plant Pathol. 2002;3:111-118.
Ramesh R, Achari GA, Gaitonde S. Genetic diversity of Ralstonia solanacearum infecting solanaceous vegetables from India reveals the existence of unknown or newer sequevars of Phylotype I strains. Eur J Plant Pathol. 2014;140:543-562.
Williamson L, Nakaho K, Hudelson B, Allen C. Ralstonia solanacearum race 3, biovar 2 strains isolated from geranium are pathogenic on potato. Plant Dis. 2002; 86:987-991.
Kim SH, Olson TN, Ralstonia solanacearum race 3, biovar 2, the causal agent of brown rot of potato, identiﬁed in geraniums in Pennsylvania, Delaware, and Connecticut (Abstract). Plant Dis. 2003; 87:450.
Cruz APZ, Ferreira V, Pianzzola MJ, Siri MI, Coll N, Valls M. A novel, sensitive method to evaluate potato germplasm for bacterial wilt resistance using a luminescent Ralstonia solanacearum reporter strain. Mol Plant Microbe Interact. 2014;27:277–285.
Vrisman CM, Deblais L, Rajashekara G,, Miller SA. Differential colonization dynamics of cucurbit hosts by Erwinia tracheiphila. Phytopathology. 2016; 106:684-692. Available:https://doi.org/10.1094/PHYTO-11-15-0289-R Link, ISI, Google Scholar.
Bekele B, Abate E, Asefa A. Dickinson M, Incidence of potato viruses and bacterial wilt disease in the west Amhara sub-region of Ethiopia. J Plant Pathol. 2011.93(1):149-157.
Assefa M, Dawit W, Lencho A and Hunduma T. Assessment of wilt intensity and identification of causal fungal and bacterial pathogens on hot pepper (Capsicum annuum L.) in Bako Tibbe and Nonno districts of west Shewa zone, Ethiopia. Int J Phytopathol. 2015;4:21-28.
Singh, DR, Kumar K, Birah A. Eco-friendly management modules for bacterial wilt (Ralstonia solanacearum) of tomato for protected cultivation in a tropical island ecosystem. Biological Agriculture and Horticulture: An International Journal for Sustainable Production Systems; 2014a.
Getachew A, Chemeda F, Seid A, Wydra K. Effects of soil amendment on bacterial wilt caused by Ralstonia solanacerum and tomato yields in Ethiopia. J Plant Prot Res. 2011;51(1):72–76.
Singh AK, Singh RK, Singh AK, Singh VK, Rawat SS, Mehta KS, Kumar A, Gupta MK, Thakur S. Bio-mulching for ginger crop management: traditional ecological knowledge led adaptation under rainfed agroecosystems. Indian J Tradit Know. 2013;13(1):111–122.
Liu Y, Shi J, Feng Y, Yang X, Li X, Shen Q. Tobacco bacterial wilt can be biologically controlled by the application of antagonistic strains in combination with organic fertilizer. Biol. Fertil. Soils. 2013; 49:447–464.
Tijjani A, Bashir KA, Mohammed I, Muhammad A, Gambo A and Habu M. Biopesticides for pests control: A review. J Biopest Agric. 2016;3(1): 6–13.
Michel VV, Mew TW. Effect of soil amendment on the survival of Ralstonia solanacearum in different soils. Phytopathology. 1998;88:300–305.
Michel VV, Wang JF, Midmore DJ, Hartman GL. Effects of intercropping and soil amendment with urea and calcium oxide on the incidence of bacterial wilt of tomato and survival of soil- borne Pseudomonas solanacearum in Taiwan. Plant Pathol. 1997;46: 600–610.
Manjunatha SB, Biradar DP, Aladakatti YR. Nanotechnology and its applications in agriculture: A review. J Farm Sc. 2016; 29(1):1-13.
Lazarovits G, Tenuta M, Conn KL. Organic amendments as a disease control strategy for soilborne diseases of high-value agricultural crops. Austr Plant Pathol. 2001;30(2):111-117.
Kago EK, Kinyua ZM, Maingi JM, Okemo PO. Influence of organic and inorganic soil amendments on soil pH and macronutrients. Journal of Agriculture and Ecology Research International. 2019;1-10.
Jaetzold R, Schmidt H, Hornetz B, Shisanya C. Central Kenya. Agroecological zones and subzones. Ministry of Agriculture, Farm Management Hand book of Kenya. Natural conditions and Farm Management information 2nd Edition Part B, Central Province: 2006;II:434-438.
Mwaniki PK, Birech R, Wagara IN, Kinyua ZM and Freyer B. Distribution, Prevalence and Incidence of Potato Bacterial Wilt in Nakuru County, KENYA. Inter J of Innov Res and Dev. 2016;5(1).
Brown PD, Morra MJ. Control of soil-borne plant pests using glucosinolate containing plants. Adv Agron. 1997;61:167-231.
Matthiessen JN, Kirkegaard JA. Biofumigation for managing soil-borne pests - progress, pitfalls and prospects. In: Zalucki M, Drew R, White G. eds. Proceedings of the 6th Australasian Applied Entomology Research Conference. University of Queensland. 1998;1:364- 372.
Heyman F, Lindahl B, Persson L, Wikstrom M and Stenlid J. Calcium concentrations of soil affect suppressiveness against Aphanomyces root rot of pea. Soil Biol. Biochem. 2007;39:2222-2229.
Gruver LS, Weil RR, Zasada IA, Sardanelli S and Momena B. Brassicaceous and rye cover crops altered free-living soil nematode community composition. Appl Soil Ecol. 2010;45:1-12.
Hartman GL, Hong WF, Hayward AC. Potential of biological and chemical control of bacterial wilt. In: Hartman GL, Hayward AC. eds. Bacterial wilt. Proceedings of ACIAR conference, 1992, Kaohsiung, Taiwan. ACIAR Proceedings No. 45. Brisbane, Australia, Watson Ferguson and Company. 1993;322-326.
Bailey KL, Lazarovits G. Suppressing soil borne diseases with residue management and organic amendments. Soil and Tillage. 2003;72(2):169-180.
Schonfeld J, Gelsomin A, van Overbeek LS, Goris-sen A, Smalla K, van Elsas JD. Effects of compost addition and stimulated solarisation on the fate of Ralstonia solanacearum biovar 2 and indigenous bacteria in soil. FMES Microbiology Ecology. 2003;43:63-74.
Kim SG, Hur OS, Ro NY, Ko HC, Rhee JH, Sung JS, Ryu KY, Lee SY, Baek HJ. Evaluation of resistance to Ralstonia solanacearum in tomato genetic resources at seedling stage. Plant Pathol J. 2016; 32(1):58-64.
Peralta IE, Spooner DM. History, origin and early cultivation of tomato (Solanaceae). In: Razdan MK, Mattoo AK, eds. Genetic improvement of solanaceous crops. Tomato. Enfield, NH:Science Publishers. 2007;2:1–27.
Maruti J. Dhanavade CB. Jalkute KD, Sonawane K, Jai SG. Study antimicrobial activity of lemon (Citrus lemon L) peel extract. Br J Pharmacol Toxicol. 2011;2 (3):119-122.
Li Y, Feng J, Liu H, Wang L, Hsiang T, Li X, Huang J. Genetic diversity and pathogenicity of Ralstonia solanacearum causing tobacco bacterial wilt in China. Plant Dis. 2016;100:1288–1296.
Rodrigues L, Destefano S, da Silva M, Costa G, Maringoni AC. Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests. J Plant Pathol. 2012; 94:505–516.
Morais TP, Lopes CA, Tebaldi ND, Luz JMQ. Occurrence and diversity of Ralstonia solanacearum populations in Brazil. Biosci. J. 2015;31:1722–1737.
Fujiwara K, Aoyama C, Takano M, Shinohara M. Suppression of Ralstonia solanacearum bacterial wilt disease by an organic hydroponic system. J Gen Plant Pathol. 2012;78:217–220.
Aslam MN, Mukhtar T, Hussain MA, Raheel M. Assessment of resistance to bacterial wilt incited by Ralstonia solanacearum in tomato germplasm. J Plant Dis Protect. 2017;124:585–590.
Sugimoto T, Watanabe K, Yoshida S, Aino M, Furiki M, Shiono M. Field application of calcium to reduce phytophthora stem rot of soybean, and calcium distribution in plants. Plant Dis. 2010;94:812–819.
Jiang JF, Li JG, Dong YH. Effect of calcium nutrition on resistance of tomato against bacterial wilt induced by Ralstonia solanacearum. Eur J Plant Pathol. 2013; 136:547–555.
Mondal B, Bhattacharya I, Khatua DC. Crop and weed host of Ralstonia solanacearum in West Bengal. J Crop Weed. 2011;7(2):195-199.