In vitro Evaluation of PHYTOSANEB-020, A Biopesticide for the Control of Mealybug (Planococcus ficus) on Rosa multiflora and Bougainvillea spectabilis in Kimwenza-Mission
A. B. Mujinga
Laboratoire de Bioénergies, Faculté de Sciences et Technologies, Université Loyola du Congo, B.P. 3724, République Démocratique du Congo.
J. Konga
Laboratoire de Bioénergies, Faculté de Sciences et Technologies, Université Loyola du Congo, B.P. 3724, République Démocratique du Congo.
B. K. Gusemba
Laboratoire de Bioénergies, Faculté de Sciences et Technologies, Université Loyola du Congo, B.P. 3724, République Démocratique du Congo.
M. K. Okito
Laboratoire de Bioénergies, Faculté de Sciences et Technologies, Université Loyola du Congo, B.P. 3724, République Démocratique du Congo.
C. N. Kuma
Laboratoire de Bioénergies, Faculté de Sciences et Technologies, Université Loyola du Congo, B.P. 3724, République Démocratique du Congo.
F. F. Kasanza
Laboratoire de Bioénergies, Faculté de Sciences et Technologies, Université Loyola du Congo, B.P. 3724, République Démocratique du Congo.
E. M. Tshiyombo
Laboratoire de Bioénergies, Faculté de Sciences et Technologies, Université Loyola du Congo, B.P. 3724, République Démocratique du Congo.
G. W. Luhata
Laboratoire de Bioénergies, Faculté de Sciences et Technologies, Université Loyola du Congo, B.P. 3724, République Démocratique du Congo.
P. L. Luhata *
Laboratoire de Bioénergies, Faculté de Sciences et Technologies, Université Loyola du Congo, B.P. 3724, République Démocratique du Congo and Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo 102-8554, Japan.
*Author to whom correspondence should be addressed.
Abstract
This study focused on the in vitro evaluation of the effectiveness of Phytosaneb-020, a bio-pesticide on Mealybug Planococcus ficus (Signoret) which infests Rosa multiflora (Rosa polyantha) and Bougainvillea spectabilis. Three treatments were used: Phytosaneb-020, Pacha a chemical insecticide and aqueous solution (distilled water) as a negative control. A volume of 1.5 mL for an area of 56.72 cm2 corresponding to the area of a Petri dish, used for each treatment. Populations of Maconellicoccus hirsutus were carefully collected from bougainvillea leaves and roses. Pacha gave the highest mortality percentage for direct toxicity by contact (98.3± 2.89%) followed by Saneb-020 (93.3 ± 7.64%). The negative control, distilled water, was not effective against the insects. No death was observed (0%). The repellency effect assay revealed a repellent activity of 80% ± 20 for Saneb-20. Distilled water gave the best avoidance effect (86.7±11.6%) pointing out the fact that Mealybug Planococcus ficus doesn’t support the presence of water. Pasha exhibited low repellency effect on Mealybug Planococcus ficus (46.7±41.6%). Further investigations on different pests are strongly suggested and encouraged to improve the effectiveness of this biopesticide.
Keywords: Mealybug, Rosa multiflora, Bougainvillea spectabilis, Phytosaneb-020; Biopesticide.